You are inserting your gene of interest into the Lac Z gene in a plasmid also containing a tetracycline resistant gene. You plate your transformed bacteria on media containing tetracyline and X-gal (the LacZ substrate that can form a blue dye). What results would indicate a clone with recombinant plasmids?

A white colony in tetracyline plates

Explanation:

Beta-galactosidase (also known as beta-gal or β-gal), will not be produced if a gene was cloned into the Lac Z gene. It is because β-galactosidase is produced by the activation of the lacZ gene, and thereby β-gal can not be produced in the absence of this lac operon. In consequence, X-gal will not be hydrolyzed by the β-gal enzyme in a reaction that releases a blue pigment, and thus the colony will be white. In gene cloning, X-gal enables to determine whether a cell is expressing a functional β-gal enzyme, which is known as the "blue/white screening method".

answer: the correct option is d. enzyme

lactase and carbonic anhydrase are the examples of enzymes.

lactase is an enzyme, which is present in the small intestine of mammals. it in the digestion of the disaccharide, lactose ( milk sugar) by converting it into two monosachharide, which are glucose and galactose.

carbonic anhydrase is present in the red blood cells. this enzyme plays significant role in the transport of co₂. it in the conversion of co₂ and h₂o into carbonic acid, which is then dissociated into bicarbonate ions and hydrogen ions.