As a way to reduce the number of cases pf malaria a human tropical disease a specific sequence is inserted into the reproductive cells of anopheles mosquitoes which process was most likely used to alter these mosquitoes

Mosquito transgenesis

Explanation:

The malaria is caused by the malarial parasite, which is transferred to the human or animal host by a mosquito bite named Anopheles. Mosquito transgenesis involves the genetic modification by introducing genes or novel elements inside the mosquito genome of the reproductive cells of the mosquito, which could prevent the replication of mosquito genome and production of progeny mosquitoes. As these mosquitoes can act as a vector for the transmission of malarial parasite, and can spread disease.

from the original order (top to bottom) the new order will go

1 (founder)

4 (hawk)

2 (mutate)

3 (next generation)