Why is it important to perform a restriction digest after cloning your gene of interest fragments into the TOPO vector?

Aneuron is stimulated with an electric pulse. the action potential is detected at a point 3.70 cm down the axon 0.0052 s later.when the action potential is detected 7.20 cm from the point of stimulation, the time required is 0.0063 s. what is the speed of the electric pulse along the axon? (why are two measurements needed instead of only one? )

Astudent sees several ants walking up a wall following the exact same trail that an ant took earlier. she wants to apply the scientific method to determine how the ants detected the trail. which of these steps would come first in her application of the scientific method? perform an experiment by cleaning the scent away from part of the trail. draw a conclusion that the ants follow a scent trail. make a prediction about what the ants will do after she cleans away part of the trail. hypothesize that the ants are following a scent trail that the first ant left.

Can happen anywhere along the spine if the neural tube does not close all the way. the backbone that protects the spinal cord does not form and close as it should. this often results in damage to the spinal cord and nerves. a) cleft palate b) spina bifida c) encephalocele d) down's syndrome