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You would expect 25 Kcal of energy stored in trophic level 4.

Explanation:

Energy flow in the ecosystem follows 10% law according to which 10% of the energy in the lower trophic level is  transferred to the next trophic level.

In this example grass belongs to the first trophic level, producers.

Energy of grass=25000Kcal

Energy transferred to trophic level 2=10% of 25000=2500

10% of the energy in trophic level 2 is transferred to trophic level 3.

Hence energy at trophic level 3=10% of 2500=250Kcal

Energy transferred to trophic level 4=10% of energy in trophic level 3

                                                              =10% of 250=25Kcal

Energy to be stored in trophic level 4=25Kcal

There will be 25 Kcal

Explanation:

The 5th - .01% out of 25000 is 2.5

The 4th -  .1% out of 25000 is 25

The 3rd - 1% out of 25000 is 250

The 2nd - 10% out of 25000 is 2,500

The 1st - 100% out of 25000 is 25,000