Calculate the volume of plasmid and insert needed for a ligation reaction using the specifications for NEBL T4 DNA ligase (0.020 pmol vector, 0.060 pmol insert). This reaction is ligating a 3kb vector (25ng/μL) and a 2kb (10ng/μL) insert together, and the fragments have complementary sticky ends. Round final answers to the nearest hundredths place.

The correct answer -

1. volume of the plasmid: 2 ml

2. insert needed = 100 mg

Explanation:

Calculating the inserted by the formula:

for a mormal vector size using 50 mg of vector DNA per ligation reaction.

take x as required insert:

3x = 300

x = 100 mg

Volume of plasmid vector is :

volume of plamid vector = 2 ml.

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