When using enzyme-linked immunosorbent assay to determine relatedness of species, what parameter is used to determine results?

If we use blank to build a roof of the house and the temperature inside the house were the main c if we use to build the roof of the house, then the temperature inside the house will remain cooler. if we use to build the walls and floors of the house then the temperature inside the house will remain cooler. if we use to bring electricity into the home then it will allow electricity to flow into the home at a faster rate . if we use to construct the latches on the windows and doors then the magnetism will keep the latches secure.