Describe how bacteria influence the GI tract.

The human GI tract is a complex system that starts from the oral cavity, continues through the stomach and intestines, and finally ends at the "happy zone". The density and composition of the microbiome change along the GI tract, with major populations being selected by the functions performed at the various locations. Bacteria along the GI tract have several possible functions, many of which are beneficial for health including vitamin production, the absorption of ions (Ca, Mg, and Fe), protection against pathogens, histological development, enhancement of the immune system, and the fermentation of “non-digestible foods” to short chain fatty acids (SCFA) and other metabolites. The roles of fungi and viruses have not been examined in as much detail; however, they are known to play important roles in microbiota dynamics and host physiology/immunity related to health and disease.

Explanation:

this protocol describes a detailed procedure for intracellular flow cytometry staining.