You are tasked with determining the number of (culturable) bacteria in soil collected from the community garden. You perform the dilution scheme below. How many bacteria are there in 1 gram of soil? Use the space below to show your work.

You are handed a streak plate and asked to provide an estimate of the number of bacteria in an individual colony. You are told that colonies typically contain 107-109 bacterial cells.

Design a dilution scheme that would enable you to estimate the number of bacteria in the colony using the quantitative plate count approach. Draw your dilution scheme below, including all plates, tubes, and volumes. Be sure to indicate which tubes would be plated (and the amount plated). Indicate the IDF and TDF for each tube.