Biology, 12.10.2020 21:01 mariahcrook7
You need to determine the sequence of a linear decapeptide. The following experiments have been performed:
1. Edman degradation generates 10 amino acids: Q, E, M, R, K, C, F, P, Y, A.
2. Cyanogen bromide (CNBr) cleavage gives two pentapeptides (5 aa’s).
3. Trypsin cleavage gives two tripeptides (AMR and QFK), and a tetrapeptide.
4. Chymotrypsin cleavage gives a dipeptide QF, a single amino acid, and a heptapeptide (7).
5. The C-terminal residue is identified as Cys using carboxypeptidase C, which removes amino acids from the C-terminal end of a polypeptide.
Give two possible sequences for the decapeptide, using aa three-letter codes.
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You need to determine the sequence of a linear decapeptide. The following experiments have been perf...
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