Describe how a lysosome enzyme would normally be created, including the steps involved and the organelles that contribute to the process

Lysosomal enzymes are produced in the ER and transported to the Golgi complex to be finally incorporated into acidified vesicles

Explanation:

Lysosomes organelles are generated by the fusion of vesicles from the Golgi complex with endosomes. This organelle contains more than 50 types of hydrolytic enzymes capable of breaking down different macromolecules. Lysosomal enzymes are produced in the endoplasmic reticulum (ER), and then they are exported to the Golgi complex, where mannose-6-phosphate (M6P) label is added to be finally packaged into acidified vesicles. Mutations in the genes that encode for lysosomal enzymes are known to produce Lysosomal Storage Diseases (LSD) including Tay-Sachs disease.

this protocol provides a detailed procedure for agarose-based ip to purify and enrich the protein of interest.

the sun's light combined with the visible wavelenghts form what is called ultraviolet radiation, which are the long waves at the end of a visible light sprectrum.

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