Your student is trying to create a "Gene X" knockout mouse by introducing a recombinant construct into embryonic stem (ES) cells, selecting transformants, and introducing the transformed cells into mouse blastocysts. He designed his construct as follows: NeoR region of homology to Gene X---HSV tk--- region of homology to Gene X where Neo is the neomycin-resistance gene and HSV tk is the Herpes Simplex virus thymidine kinase gene. He transforms the ES cells and exposes them to G418 and ganciclovir. Explain to your student why he failed to recover transformed ES cells. Choose the two correct statements. a. His construct incorporated into the mouse DNA by illegitimate recombination. These cells would be sensitive to ganciclovir and neomycin, and would die.
b. His construct incorporated into the mouse DNA by illegitimate recombination. These cells would be resistant to ganciclovir, but sensitive to neomycin, and would die.
c. His construct incorporated into the mouse DNA by homologous recombination. These cells would be sensitive to ganciclovir, but resistant to neomycin, and would die.
d. His construct incorporated into the mouse DNA by homologous recombination. These cells would be sensitive to neomycin and ganciclovir, and would die.
e. His construct incorporated into the mouse DNA by homologous recombination. These cells would be sensitive to neomycin, but resistant to ganciclovir, and would die.
f. His construct incorporated into the mouse DNA by illegitimate recombination. These cells would be resistant to neomycin, but sensitive to ganciclovir, and would die.

answer:

the water molecules are transferred from the roots to the stem or top parts of the tall tree because of important properties of water which are adhesion, cohesion and capillary action. cohesion is the property of water molecules keeps water molecules in a combined state by sharing of bonds between them at the atomic level. adhesion allows the interaction of water molecules with other substances or substrate. adhesion will cause the attachment of water molecules to the wall of the vessel (xylem) of the plant. capillary action occurs when the adhesive force to the vessel wall is stronger than the cohesive force between water molecules. capillary action mimics the transfer of water from a tube or pipe. in this way water utilizes its property to transfer water from down to top level of the plant.  

an individual’s sex (i.e., whether they are a male or female) is determined by the sex chromosomes. most people have two sex chromosomes, one that is inherited from their mother and one that is inherited from their father. typically, females have two x chromosomes (xx) and males have one x chromosome and one y chromosome (xy). conditions caused by changes (“mutations”) in genes located on the x chromosome are considered x-linked.

x-linked recessive inheritance

most x-linked conditions are recessive. this means that in a person with two x chromosomes (most females), both copies of a gene (i.e., one on each x chromosome) must have a change or mutation whereas in a person with one x chromosome (most males), only one copy of a gene must have a mutation. a female with a mutation in one copy of a gene on the x chromosome is said to be a “carrier” for an x-linked condition. a male with a mutation in a gene on the x chromosome is typically affected with the condition. because females have two copies of the x chromosome and males have only one x chromosome, x-linked recessive diseases are more common among males than females. however, x-linked recessive diseases can occur in both males and females.

for x-linked recessive disorders, an unaffected carrier mother who has a mutation in a gene on the x chromosome can transmit either the x chromosome with this mutation or a “normal” x chromosome to her children. if the father is unaffected, none of her daughters will be affected and all of her daughters will be unaffected—since they will inherit at least one normal x chromosome from their father. however, each daughter will have a 50% chance of being an unaffected carrier like her mother and a 50% chance of both x chromosomes being normal.

that would be called plucking : )

explanation:

''plucking, also referred to as quarrying, is a glacial phenomenon that is responsible for the erosion and transportation of individual pieces of bedrock, especially large "joint blocks". as a glacier moves down a valley, friction causes the basal ice of the glacier to melt and infiltrate joints (cracks) in the bedrock."