Biology, 12.08.2020 07:01 brook633

Aliquots of a 7.8-kilobase (kb) linear piece of DNA are digested with the restriction enzymes PvuII, HincII, ClaI, and BanII, alone and in pairs. The digestion products are separated by gel electrophoresis, and the size of each fragment is determined by comparison to size standards. The fragment sizes obtained, in kilobase pairs, are given below. Draw a diagram of the 7.8-kb fragment, showing the location of the restriction sites for each enzyme. a. Digestion with PvuII: 1.3 kb, 6.5 kb
b. Digestion with HincII: 0.5 kb, 3.3 kb, 4 kb
c. Digestion with ClaI: 1 kb, 6.8 kb
d. Digestion with BanII: 1.3 kb, 6.5 kb
e. Digestion with PvuII and ClaI: 1 kb, 5.5 kb, 1.3 kb
f. Digestion with PvuII and HincII: 0.5 kb, 0.8 kb, 2.5 kb, 4 kb
g. Digestion with HincII and ClaI: 0.5 kb, 1 kb, 3 kb, 3.3 kb
h. Digestion with HincII and BanII: 0.5 kb, 0.8 kb, 2.5 kb, 4 kb
i. Digestion with ClaI and BanII: 1 kb, 1.3 kb, 5.5 kb

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Aliquots of a 7.8-kilobase (kb) linear piece of DNA are digested with the restriction enzymes PvuII,...

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