Place the 4 steps of biofilm formation, listed below, in the correct order. 1. Surface (substratum) is preconditioned by environmental molecules.
2. Quorum sensing and the establishment of the extracellular matrix commences as microbes attach more stably.
3. Biofilm matures and some microorganisms escape to the planktonic state.
4. Microbes attach and detach from the preconditioned surface.

1. Surface (substratum) is preconditioned by environment molecules.

4. Microbes attach and detach from the preconditioned surface.

2.Quorum sensing and the establishment of the extracellular matrix commences as microbes attach more stably.

3. Biofilm matures and some microorganisms escape to the plank-tonic state.

Explanation:

Biofilm is a process in which microorganism irreversibly attach and grow on the surface to produce extra cellular polymers that facilitate formation of matrix. The biofilm process takes three days for the formation after which thickness of plaque increases. There are 4 main steps in biofilm formation. Surface is preconditioned by environment molecules. The microbes attach the preconditioned surface. Establishment of extra cellular matrix and finally biofilm matures.

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