Based on your knowledge of the genetic tools for studying baker's yeast, how would you clone the genes that are mutated in your respective yeast strains?

By using specific primers for PCR amplification and DNA sequencing

Explanation:

In molecular biology, different techniques are used to clone the full sequence of desired genes. In general, gene cloning is based on the assembly of genetically engineered recombinant DNA cloning vectors into host organisms. The cloning procedure generally consists of isolating target DNA sequences to be cloned, ligation into a corresponding vector, transfection and screening of clones. The recombinant DNA is first amplified by PCR. Subsequently, DNA is sequenced and finally assembled by bioinformatic methods in order to characterize the full sequence of the gene.

[content deleted] - user123802726recklesschicken

an active site is a region that is present on an enzyme where the reactants or the substrate molecules we can say bind and experience a chemical reaction. the substrate is basically a reactant whose concentration is changing and that is converting into a product after binding at the active site of an enzyme