3. Mutations that occur in the BPG binding cavity on deoxyhemoglobin can at times result in the substitution of lysine and histidine residues present with aspartic acid and phenylalanine residues. Explain how these changes would impact BPG binding to deoxyhemoglobin along with the impact it would have on oxygen transport with hemoglobin

It affects the oxygenation of the tissues in a negative way, that is, causing tissue hypoxias.

Explanation:

What will happen is that BPG, having a different relationship with deoxyhemoglobin, hemoglobin will not give oxygen to organs or tissues during glycolysis at times when the sympathetic nervous system is activated or even in the maintenance of physiological tissue vitality, therefore it will trigger a drop in the partial pressure of the gas both in the blood and in the tissues since they will be retained in the hemoglobin structure without being able to release it.

the answer is; comparing the frequency of collisions with and without the device

this makes the anti-collision system an independent variable and the collisions a dependent variable. if there are fewer collisions with an anti-collision system that without, then it can be stated that the system is a success. essentially, collisions under an anti-collision system are compared with the frequency of collisions by cars with the human drivers.