Why KOH solution is kept in the test tube inside the air tight conical flask while doing the experiment of respiration of seeds?

Cholera is a bacterial infection of the small intestine. vaccination provides protection against cholera. the cholera vaccine is made up of weakend form of the bacteria that causes cholera. the vaccine will stimulate blank production and elicit an immune response. if the infection occurs again, the bodynwill produce an even greater attack on thre bacteria because of blank.