1. List the DNA strand sequence from which the mRNA was transcribed:

2. List the complementary DNA sequence to the above DNA strand:

3. List the tRNA sequence that is complementary to the mRNA strand above:

4. Using the codon wheel, list the amino acid sequence of the protein coded for by the original mRNA sequence:

5. a) What would be the effect of changing base 4 to a G? b) What would the effect be if base 6 was changed to a G? c) What would be the effect of changed base 16 to a U?

d) What would happen if base 7 was deleted?

e) What would the new amino acid sequence be?

Explanation:

1. DNA sequence-

2. Complimentary DNA sequence-

3. tRNA sequence to mRNA- CAU-UAC-UUU-GCG-GAC-CAU-CUU-CCA-ACU-ACG.

4. Ammo acid sequence- met-lys-arg-leu-val-glu-gly

5. a) What would be the effect of changing base 4 to a G?

This would change AUG to GUG changing the start codon and not allowing for the translation of the mRNA unless it encounters another start codon AUG

b) What would the effect be if base 6 was changed to a G?

There would be no effect as it still remains as a G

c) What would be the effect of changed base 16 to a U?

This would change GUA to UUA and this codon specifies for the amino acid leucine in place of valine thus changing the amino acid.

d) What would happen if base 7 was deleted?

If base 7 was deleted, there would be a chance in the mRNA frame and subsequently a change in the amino acid sequence.

e) What would the new amino acid sequence be?

Met-Asn-Ala-Trp