Predict how the following environmental changes will affect the pKa of a H residue in a protein: a) a D residue in the same protein is brought into close proximity pKa b) the H side chain shifts from the outside of the protein to a nonpolar site inside pKa c) an R residue in the same protein, that was close, moves away. pKa In each case, will the pKa go up, down, or remain the same? Explain. You explain for each.

Taq polymerase is an enzyme isolated from the organism thermophilus aquaticus. this organism has been found living in the hot springs of yellowstone national park. this enzyme is used to copy human dna from crime scenes. most reactions are performed at ranges similar to those of the human body; however, what considerations should be made for optimum use of this enzyme? view available hint(s)taq polymerase is an enzyme isolated from the organism thermophilus aquaticus. this organism has been found living in the hot springs of yellowstone national park. this enzyme is used to copy human dna from crime scenes. most reactions are performed at ranges similar to those of the human body; however, what considerations should be made for optimum use of this enzyme? the enzyme will not work on human dna.nothing should be altered.the ph should be decreased.the temperature should be raised.