A DNA sequence of interest was digested and ligated into the middle of the AmpR gene region of pBR322 vector. The vector also contains a TetR gene which is not interrupted. After introducing the recombinat DNA into bacterial cells, the resulting transformants were tested on a variety of media. Which of the following results would be expected? Group of answer choices Growth on tetracyclin containing media. Death on media containing both tetracyclin and ampicillin. Growth on media containing both tetracyclin and ampicillin. Growth on ampicillin containing media.

The following two results are possible:

Option A "Growth on tetracyclin containing media." and

Option B "Death on media containing both tetracyclin and ampicillin."

Explanation:

Since the TetR gene was not interrupted, the bacteria is resistant to the presence of Tetracyclin antibiotic in the media, so yes, it will grow when there is only tetracyclin present. However, when ampicillin is also introduced, the bacteria can no longer grow since its AmpR gene has been rendered non-functional. It is now no longer resistant to the presence Ampicillin and hence, will not be able to grow in its presence.

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