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Biology, 05.05.2020 23:26 lychee1406
From a protein assay, you determine that your sample of purified protein contains 3 mg protein/mL. You need to prepare a sample for SDS-PAGE. The final volume of the sample for SDS-PAGE should be 24μL and the final concentration of sample buffer in the SDS-PAGE sample should be 1X. You would like to load a total of 15μg of your purified protein on the gel. Determine the volume of the purified protein, volume of 6X sample buffer, and volume of water needed to prepare a sample for SDS-PAGE (recall final volume should be 24μL).
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From a protein assay, you determine that your sample of purified protein contains 3 mg protein/mL. Y...
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