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You hypothesize that a transcriptional regulator may control expression of specific genes in LB medium, and want to find out what these genes are. You have made a set of strains (i. e., library) in which a promoterless lacZ was inserted at varied chromosomal locations. Design an experiment to identify these genes using agar plates with LB and X-gal. Describe the steps in this procedure.
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You hypothesize that a transcriptional regulator may control expression of specific genes in LB medi...
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