You are asked to give a classroom demonstration of DNA supercoiling. To do so, you take two different colored pieces of rubber tubing, twist them very tightly and excessively around each other and join the ends of the tubing appropriately. Next, you remove the supercoiling by breaking one strand and unwinding it. In this demonstration, the breaking and winding simulates the action of___.

topoisomerases

Explanation:

Topoisomerases are enzymes that help overwind or underwind DNA. DNA double helix has high tedency to get winded again because of its structural characeristics.

During replication and transcription of DNA, DNA gets overwound in front of a replication fork. This enzyme stops the double helix of DNA from becoming too tightly bound in front of the replication fork as the DNA opens.

The given demonstartion shows the action of topoisomerases as it shows unwinding of of supercoiled strands of pipe (DNA), which is don eby topoisomerases in the DNA replication process.

Hence, the correct answer is topoisomerases.

d.

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enzyme active site and substrate specificity

enzymes bind with chemical reactants called substrates. there may be one or more substrates for each type of enzyme, depending on the particular chemical reaction. in some reactions, a single-reactant substrate is broken down into multiple products