C. (1) A new form of Cry1A protein has been developed that targets the mutated cadherin protein and confers resistance to mutant rootworm genotypes. Order the following steps, (1 through 5) to introduce the gene for the new Cry1A protein into an elite corn hybrid. Answer using numbers 1, 2, 3, 4, 5 Test for expression of the gene in transgenic plants Regenerate plants carrying the new gene by tissue culture Create a construct carrying the gene using recombinant DNA technologies Transform the construct carrying the gene into corn plant cells Move the gene into high-performing varieties

1) Create a construct carrying the gene using recombinant DNA technologies.

2) Transform the construct carrying the gene into corn plant cells.

3) Regenerate plants carrying the new gene by tissue culture.

4) Test for expression of the gene in transgenic plants.

5) Move the gene into high-performing varieties by conventional breeding.

Explanation:

relationships aren't everything. people everyday spend there entire life depending on reltionships and for someone to be there for them when this is absurd. why must people depend on someone else to bring them happiness? i'm not saying it wrong to feel happy with someone, but people feel that they need to be in a relationship in order to feel happy, when, this is not true. you can paint, write, read ir slend tume with family ir even spend time working in yourself. this can be an exciting experience to find yourself. it's bot wrong to be in a relationship but if you let it be your only source of happiness, than you will never find true happiness.

so, relationships are not always worth it. if you are so dependent on them, than in the end they arent worth it because you will realize how unhappy you really were. some people even tend to cut ties with family because they think they cabt make them happy, obly realtionships with a lover can. false. in order to find true happiness, one must learn to love themselves if they want to love someone else. it's unhealthy to be always depend on relationships and to alqays be in and out of one.

so live life, get that job you always wanted, buy those new shoes and love yourself. depend on yourself, not others. they arent worth it, you can make yourself more happier than most people can do to you. it's not woth it. take your time and have fun.

characterization of nanoparticles is a critical step in nanomaterial production as it provides information of dimension, morphology, surface charge, distribution and degree of surface functionalization.