1. Makes cells permeable to plasmid DNA

2. Selects for the growth of transformed cells

3. Provides the GFP gene

4. Induces GFP gene expression

5. Causes Green Fluorescent Protein to fluoresce

A. CaCl2

B. Ampicillin

C. pGLO plasmid

D. Arabinose

E. UV Light

1 can be correctly matched with A, Calcium chloride enhances the permeability of plasmid DNA within the cell.  

2 can be correctly matched with B, only the ampicillin-resistant cells or the transformed cell possesses the tendency to grow in the selective media.  

3 can be correctly matched with the pGLO plasmid, the isolation of GFP was done originally from jellyfish, and is usually administered within the pGLO plasmid for examining the expression.  

4 can be correctly matched with D, the expression of GFP is instigated by arabinose as a bidirectional promoter is shared by GFP with the arabinose gene, and thus can get stimulated in its existence.  

5 can be correctly matched with E, in the presence of ultraviolet light, the green fluorescent protein gets fluorescent.  

both anaerobic and aerobic respiration use glucose (or other carbohydrates), proteins and lipids to produce atp and nadh. cell uses those energy molecules, obtained from food and   breaks them down during the chemical reactions of respiration. in anaerobic respiration, nadh is regenerated to nad+ by enzymes lactate dehydrogenase (lactate is produced, e.g. in muscle cells) or by alcohol dehydrogenase where ethanol is produced (alcohol fermentation), while in aerobic respiration, electrons from nadh are deposited in the mitochondria to regenerate nad+ (electron transport chain. anaerobic respiration produces 2 atp per molecule of glucose compared to aerobic respiration where about 38 atp molecules are produced.

the oxygenated blood then leaves the lungs through pulmonary veins, which return it to the left heart, completing the pulmonary cycle. this blood then enters the left atrium, which pumps it through the mitral valve into the left ventricle. from the left ventricle, the blood passes through the aortic valve to the aorta.

deoxygenated blood leaves through the right ventricle through the pulmonary artery. from the right atrium, the blood is pumped through the tricuspid valve (or right atrioventricular valve), into the right ventricle.