Describe a strategy using restriction enzymes and gel electrophoresis that would allow you identify that you have obtained the Recombinant DNA plasmid (containing YFG). Be specific indicating the techniques you would use and expected results.

Mike was adopted, and his biological family history is unknown. although he is healthy, he would like some understanding of his genetic makeup, including potential health risks and genes that he could pass on to his children. mike has heard about commercial laboratories that can compare segments of your dna to those of people with common hereditary diseases in order to give you some idea of how susceptible you are to the diseases. the results of these types of tests are highly inconclusive. if mike's dna showed that he shares similarities in a segment of dna with people who have a given disease, his chances of developing that disease or passing it on to his children may be slightly elevated, but they are not 100 percent. these tests can cost more than $2,500 and are not covered by many insurance companies. do you think that mike should undergo the genetic tests? explain your answer. what are the potential pros and cons of having such tests done? describe how the availability of these genetic tests might affect the frequency of genetic diseases in individuals and populations.

Where does all the water go? according to the environmental protection agency (epa), in a typical wetland environment, 39% of the water is outflow; 46% is seepage; 7% evaporates; and 8% remains as water volume in the ecosystem (reference: united states environmental protection agency case studies report 832-r-93-005). chloride compounds as residuals from residential areas are a problem for wetlands. suppose that in a particular wetland environment the following concentrations (mg/l) of chloride compounds were found: outflow, 60.4; seepage, 73.7; remaining due to evaporation, 26.4; in the water volume, 46.8. (a) compute the weighted average of chlorine compound concentration (mg/l) for this ecological system. (round your answer to one decimal place.) mg/l (b) suppose the epa has established an average chlorine compound concentration target of no more than 58 mg/l. does this wetlands system meet the target standard for chlorine compound concentration? yes. the average chlorine compound concentration (mg/l) is too high. yes. the average chlorine compound concentration (mg/l) is lower than the target. no. the average chlorine compound concentration (mg/l) is lower than the target. no. the average chlorine compound concentration (mg/l) is too high.

Crossing over and independent assortment are important activities that occur during meiosis. what is the significance of these activities