What are mutations, and how can they effect proteins?

By changing a gene's instructions for making a protein, a mutation can cause the protein to malfunction or to be missing entirely. When a mutation alters a protein that plays a critical role in the body, it can disrupt normal development or cause a medical condition.

Hope this helps :P

Mutations are permanent changes in the DNA sequence of a gene. Mutations can affect the shape of the protein that is produced. A change in the shape of the protein can affect the function of the protein, which may affect the trait that is seen.

Explanation:

this protocol describes a detailed procedure for intracellular flow cytometry staining.