Suppose you made the intestinal sac too small, and at the end of experiment you could collect only 100 microliters of solution instead of 200 microliters for analysis. You perform the DNS reaction with 100 ul of the recovered solution and 100 ul of water (to bring volume up to 200 ul). How that would affect the concentration of glucose you determined from the standard curve?

Normal Experiment
200 ul solution
200 ul of DNS
400 ul of H2O

A)Half the true concentration
B)Twice the true concentration
C)Equal to the true concentration