For the organism Corynebacterium diphtheriae, the causative agent of diphtheria, drag the appropriate general characteristics of morphology, reproduction and arrangement, along with the classification grouping, to describe this organism.

Corynebacterium diphtheriae have a morphology described as . The reproductive strategy is , which causes a arrangement. The classification of this microbe is in the grouping.

A. alphaproteobacteria
B. cocci
C. staphylo- strepto- snapping division
D. betaproteobacteria
E. curved rods (vibrios)
F. low G-C gram-positive
G. budding
H. high G-C gram-positive
I. tetrad
J. blebbing

Corynebacterium diphtheriae have a morphology described as high G-C gram-positive club shaped rods. The reproductive strategy is staphylo- strepto- snapping division , which causes a palisades arrangement. The classification of this microbe is in the High G-C gram positive Actinobacteria grouping.

Explanation:

Corynebacterium contain diaminopimelic acid in their cell walls, and microscopically form palisades, or pairs of rod-shaped cells resembling the letter V. Cells may contain metachromatic granules, intracellular storage of inorganic phosphates that are useful for identification of Corynebacterium. The vast majority of Corynebacterium spp. are nonpathogenic; however, C. diphtheria is the causative agent of diphtheria, a disease that can be fatal, especially in children. C. diphtheriaproduces a toxin that forms a pseudomembrane in the patient’s throat, causing swelling, difficulty breathing, and other symptoms that can become serious if untreated.

this protocol describes a detailed procedure for intracellular flow cytometry staining.

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