A graduate student is trying to isolate bacterial mRNA for an enzyme that will degrade trinitrotoluene (TNT). She's frustrated to find that the enzyme isn't produced when the bacterium in question is grown on nutrient agar. What might she do to solve the problem and obtain the mRNA?
A. Add nitrogen and toluene to the agar to drive up synthesis of TNT. B. Add TNT to the agar-this will drive synthesis of the enzyme to degrade it, leading to production of the mRNA the student is looking for. C. Remove glucose from the agar-this will cause the bacteria to shift to other compounds for their energy purposes. D. Remove all sugars from the agar-this will cause the bacteria to shift to other compounds for their energy purposes. E. Add TNT to the agar-this will drive synthesis of the enzyme to degrade it, leading to production of the mRNA the student is looking for AND remove all sugars from the agar-this will cause the bacteria to shift to other compounds for their energy purposes.