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Biology, 30.03.2020 23:17 KayleighMorganhopkin

1. You are given the task to quantify the bacteria in your soil sample. You do a 10-fold serial dilution of your soil sample in sterile water and plate 1/10 of each subsequent (serial) dilution in LB agar plates with cycloheximide – an antifungal and eukaryotic cell inhibitor. If your 10–6 (ten to the minus sixth) dilution plate contains 97 bacterial colonies after two days of incubation, how many bacteria can you estimate were present in your initial soil sample (1 g of soil)? Show your calculation and briefly describe each step.

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