In order to add the gene for human insulin to a bacterial plasmid, the DNA molecules have to be "cut" with enzymes called restriction endonucleases and then pasted back together with enzymes called DNA ligases. Imagine that during this process, the first five nucleotides of the human insulin gene were accidentally cut out before it was pasted into the bacterial plasmid. What is the most likely outcome if this plasmid was added to bacterial cells?

Human insulin protein will not be produced

Explanation:

For production of human insulin protein in bacteria, first the insulin gene needs to be transcribed to corresponding mRNA. This mRNA will be then translated to produce insulin protein. The initial portion of any gene has a promoter sequence. During transcription RNA Polymerase recognizes this promoter sequence to initiate the process.

Here, the gene's first five nucleotides were accidentally cut out so now the promoter sequence has been altered. RNA Polymerase will not be able to recognise it so transcription will not occur. No mRNA will be formed and as a result translation will also not occur and there will be no production of insulin protein.

the relative standard reduction potential of the half-cell in which reduction occurs; more positive than the other half-cell. reduction is a chemical process in which electrons are added to an atom or an ion; it always occurs accompanied by oxidation of the reducing agent.

the cladogram shown here shows similarities and differences among several classes of vertebrates. anatomically, the four have limb structure as their common ancestor. we might separate the mammals from the other three classes because they a) divergent; do not lay eggs b) identical; are warm-blooded c) varied; a four chambered heart d) homologous; have mammary glands