How do i use prokaryote in a sentence

Consider this plant cell which organelle is labeled g

This part insulates the reaction chamber from the transfer of heat to or from the surrounding environment

According to theories of how life began, how did early organic molecules begin to separate from the outside world? a: specialized enzymes were required b: chains of amino acids created a barrier c: formation of microspheres or vesicles d: rna catalyzed the formation of membranes