. Using aseptic technique, inoculate each of the appropriately labeled media listed below in the following manner: a. Nutrient agar slants: With a sterile needle, make a single-line streak of each of the cultures provided, starting at the butt and drawing the needle up the center of the slanted agar surface. b. Nutrient agar plates: With a sterile loop, prepare a streak-plate inoculation of each of the cultures for the isolation of discrete colonies. c. Nutrient broth cultures: Using a sterile loop, inoculate each organism into a tube of nutrient broth. Shake the loop a few times to dislodge the inoculum. d. Nutrient gelatin: Using a sterile needle, prepare a stab inoculation of each of the cultures provided. 2. Incubate all cultures at 37°C for 24 to 48 hours.

1. label high-pressure areas with the letter h. 2. label low-pressure areas with the letter l. 3. over which area(s) would you expect to see rain or snow? 4. over which area(s) would you expect to see clear skies? 5. draw arrows around the h on the map to show the wind direction. 6. draw arrows around the l on the map to show the wind direction. 7. imagine that you live in maine. currently there is a high-pressure area over maine. if a low-pressure area moves in, how will the direction of the wind change? 5. draw arrows around the h on the map to show the wind direction. 6. draw arrows around the l on the map to show the wind direction. 7. imagine that you live in maine. currently there is a high-pressure area over maine. if a low-pressure area moves in, how will the direction of the wind change? 8. imagine that you live in colorado. currently there is a low-pressure area over colorado. if a high-pressure area moves in, how will the direction of the wind change? 9. according to the map, where would the strongest winds be expected? (refer to the lesson if needed.)

White-tailed deer are considered to be an overpopulated species in the central united states. which of these events probably contributed the most to white-tailed deer exceeding their carrying capacity?

Isolated volcanic peaks on the ocean floor are