During the early stages of an enzyme purification protocol, when cells have been lysed but cytosolic components have not been separated, the reaction velocity versus substrate concentration is sigmoidal. As you continue to purify the enzyme, the curve shifts to the right. Explain your results.

Aresearcher created three groups based on participants bmi: normal weight, overweight and obese. the hypothesis being tested is that the three groups differ in the mean number of artificially sweetened drinks consumed weekly. which statistical test might the researcher use, assuming a reasonably normal distribution of values.