The A260/A280 ratio method estimates protein by measuring the absorbance maximum at 280nm caused by the amino acids, , and . Since also absorbs in the UV range, we can correct for this contaminate by measuring the absorbance maximum at and using the following equation:

PurityAromatic rings, peptide bonds, primary structure, secondary structure, and tertiary structure,Nucleic acid,260nm.

Explanation:

The A260/A280 ratio method estimates protein purity by measuring the absorbance maximum at 280nm caused by the amino acids aromatic rings, peptide bonds, primary structure, secondary structure, and tertiary structure. Since nucleic acid also absorbs in the UV range, we can correct for this contaminate by measuring the absorbance maximum at 260nm and using the following equation.

Volcanoes make new rocks so not ahotspots are the the stuff that makes volcanoes mid atlantic ridge(mar cause i’m lazy) is a constructive plate boundary so it makes sense that new rocks would be found near it, also the mar is in the middle of the ocean so it fits the description deep ocean trench, umm, there’s no ocean trenches to where you’re goingso the mar is most likely the answeri wouldn’t trust me but hey why not