Why is the Mediator Complex only required for transcription in vivo, but is dispensable in vitro? What is different about the DNA template in vivo compared to the templates used in in vitro transcription systems?

i'm thinking 600 feet deep in one of the finger lakes.

answerreferred pain;

in addition to being able to metabolize, grow, digest food, and excrete wastes, what other functions must an organism perform if it is to survive? survival also depends on the ability to maintain one's boundaries, to move, to respond to stimuli, and to reproduce. oxygen is a survival need.