As a result of DNA helicase activity, topoisomerases are also required during replication. Explain how topoisomerases help DNA helicases function more efficiently. As a result of DNA helicase activity, topoisomerases are also required during replication. Explain how topoisomerases help DNA helicases function more efficiently. Topoisomerases cause formation of positive supercoils. Topoisomerases cause formation of negative supercoils. Topoisomerases prevent formation of negative supercoils. Topoisomerases prevent formation of positive supercoils.

topoisomerase prevents formation of positive supercoil.

Explanation:

During replication,at the replication fork, the helicases functions to unwind the DNA. As unwinding occurs, the DNA upstream becomes overwound/positively supercoiled and become tangled, just like experimenting with a length of rope. So, for the cell to avoid DNA tangling, the topoisomerases helps in reducing/preventing overwounding by forming breaks in the phosphate backbone of the DNA so that they rotae and become relaxed and then the topoisomerase seals up the breaks. The topoisomerases are differentiated by the number of breaks they make.

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