You have isolated a mutant strain of Bacillus subtilis (which you designate wimP) whose endospores can be killed by boiling water. When you analyze the endospores produced by this strain, you find that compared to endospores produced by the parent (a wild-type strain), the wimP spores have significantly:

A. elevated amounts of germination factors.

B. reduced amounts of small acid-soluble proteins.

C. reduced amounts of germination factors.

D. lower amounts of spore coat proteins.

E. higher water content.

The correct answer is B. reduced amounts of small acid-soluble proteins.

Explanation:

Spores of Bacillus subtilis contains very small amount of small acid-soluble proteins which only comprise of 20% of the total spore core protein. These small acid-soluble proteins provides resistance against chemicals, heat or UV radiation.

during the process of dna replication, brdu can replace thymidine and incorporate into the newly synthesized dna, cell proliferation thus can be reflected in the extent of brdu incorporation.