When dicyclohexylcarboiimide (DCCD) is added to a suspension of tightly coupled, actively respiring mitochondria, the rate of electron transfer (measured by O2 consumption) and the rate of ATP production dramatically decrease. If a solution of 2,4-dinitrophenol (DNP) is now added to the preparation, O2 consumption returns to normal but ATP synthesis remains inhibited. What process in electron transfer or oxidative phosphorylation is affected by DCCD
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When dicyclohexylcarboiimide (DCCD) is added to a suspension of tightly coupled, actively respiring...
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