Thankfully, another lab has sequenced the bacterial genome, so you know where on the bacterial chromosome the genes you are interested in are located, as well as the DNA sequences before and after them. To remove the genes from the chromosome, you will use enzymes called restriction endonucleases that will cut the DNA strand at specific base sequences. This "digestion" will allow you to tailor the DNA fragment that you will be removing from the chromosome so that you may know where the cuts will be made and what additional DNA bases may come with the fragment containing the genes you are interested in.

The cuts are that are made by endonucleases have two outcome. Either they will be of sticky ends or blunt ends. Both of these ends come as a result of type of endonuclease that we are going to use.

There are also some extra sequence fragments that came additionally after cutting procedure. For example, the endonuclease EcoR1 cut the DNA fragments from 5' end that are sticky in nature and extra fragments of AATT hang up after cutting.

lunar landing mission. apollo 11 was the first manned mission to land on the moon. the first steps by humans on another planetary body were taken by neil armstrong and buzz aldrin on july 20, 1969. the astronauts also returned to earth the first samples from another planetary body.

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answer: although many types of viruses can cause a common cold, rhinoviruses are the most common culprit. a cold virus enters your body through your mouth, eyes or nose. the virus can spread through droplets in the air when someone who is sick coughs, sneezes or talks. theres your answer

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