Arrange the following sequence of procedures in the proper order in this experiment done to determine proteins present in pea plants during germination. 1. Load the proteins in polyacrylamide gel electrophoresis and separate them 2. Break the cells by grinding them in liquid nitrogen and suspending in a buffer 3. Select a suitable tissue to isolate proteins expressed only during germination 4. Centrifuge the broken cells to purify proteins from other molecules

Which statements best describe the differences or similarities between a comparative investigation and an experimental investigation? check all that apply. experimental investigations are best for showing cause-and-effect relationships, while comparative investigations do not necessarily show this type of relationship. comparative investigations are often impractical and unrealistic, while experimental investigations are the most practical and realistic kinds of investigations. comparative investigations allow for the use of a wide range of variables but do not have a control group, while experimental investigations involve variables that can be controlled. both experimental investigations and comparative investigations are designed to answer scientific questions. both experimental investigations and comparative investigations focus on making observations rather than collecting new data.