100 µL of India ink was added to the T. vorax cultures. How would the results be affected if 20 µL were used instead?

A. The average number of food vacuoles formed in 30 min would increase.
B. The average number of food vacuoles formed in 30 min would decrease.
C. The average number of food vacuoles formed in 30 min would not be affected.

Option B,  The average number of food vacuoles formed in 30 min would decrease.

Explanation:

As we know ink solution act as a food source for the T vorax culture. The higher will is the amount of solution added, the higher will be the number of food vacuoles formed in a given time period.  

Therefore, when 20 micro liter of ink solution is added instead of 100 micro liter of ink the food vacuole number will decrease.  

Hence, option B is correct