Forward primer: 5'-AGTCTACTCGTAACCGGTTACC-3' Reverse primer: 5'-TAAGGCATCATGGTAACCGGTT-3' The two primers above have the melting temperatures between 55C-80C. What is the reason that these two primers will not bind effectively to the region of DNA that we want to amplify?a. The length of the two primers is between 20-30 base pairs long. The length is too short and not very specific. b. The 3'-end of the reverse primer (3'-TTGGCCAATGG---5') is complementary to the forward primer (5'---AACCGGTTACC-3') and thus reduces the ability of the primers to bind to the targeted DNA sequence. c. The 3'-end of the forward primer is complementary to the 5'-end of the reverse primer and thus reduces the ability of the primers to bind to the targeted DNA sequence. d. The melting temperature range of the primers is similar to the DNA. e. Guanine and Cytosine make up 60% of the total base pairs.

List two new traits (structural or behavioral) that each new species of rat might demonstrate as it adapts to the conditions on two of the four islands. choose only two islands that are described in the lesson. record the island latter and the major habitat feature of the island. then list two new traits each rat subspecies might demonstrate in order to survive the habitat on that island. note: new traits should be unique to that island and be in response to that island’s habitat feature. i cannot remember islands that were names in the lesson so only answer if you have done the lesson (5.13 life science 7b) choose 2 of the islands and describe a structural and a behavioral trait for a rat on each island so 4 total answers, 2 different structurals and 2 different behaviorals, one for each rat for each island

After getting the slide and microscope ready, stella is eager to see the microscopic pond water specimen! she crouches around her microscope and aligns her eye with the microscope’s eyepiece. but she's only able to see a bright white light. at this point, no specimens or cells are visible on stella's pond water slide. after taking a moment to collect her thoughts, stella realizes she can't see any lifeforms because .

Me plz he did not infringe upon the policy making powers that he felt the constitution gave congress. but the determination of foreign policy became preponderantly a presidential concern. when the french revolution led to a major war between france and england, washington refused to accept entirely the recommendations of either his secretary of state thomas jefferson, who was pro-french, or his secretary of the treasury alexander hamilton, who was pro-british. rather, he insisted upon a neutral course until the united states could grow stronger. which provides the best objective summary of this excerpt? president washington made a good choice when he decided not to accept the recommendations of his advisors and instead insisted that the united states stay neutral during the war between france and england as president, washington believed that the united states should remain neutral in foreign policy even though he had advisors who recommended that he take sides during the war between france and england. washington did not make policy decisions during his presidency because he did not want to interfere with the policy-making powers that the constitution gave congress. washington refused to accept entirely the recommendations of either his secretary of state thomas jefferson, who was pro-french, or his secretary of the treasury alexander hamilton, who was pro-britis