Recombinant DNA used to produce human insulin protein in bacterial cells. This procedure relied on cloning a human gene into a plasmid at a location directly adjacent to a bacterial promoter sequence. Why not use the promoter from the human insulin gene for this application?

Promoter from the human insulin gene has large intron and limited tRNAs for complete expression

Explanation:

A bacterial promoter sequence is used as it is made intron free which is not the case with human gene as it contains large introns. Hence an intron free version is created as cDNA. cDNA is made out of mRNA and has no regulatory region. Also, the bacterial promoter sequence contains sufficient ribosome-binding site, and terminator sequences with tRNAs in number enough for complete expression

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answers are:

lysogenic     viral dna merges with host cell dna

lysogenic     no symptoms

lytic               viral dna takes over

lysogenic     virus does not destroy host cell

lytic               virus destroys host cell

lytic               induces symptoms of viral infection

lysogenic       cell reproduces and spreads provirus