The kinetics analyses of an enzyme are measured as a function of substrate concentration in the presence and in the absence of 2mM inhibitor.
[S] (μM): 3, 5, 10, 30, 90
Velocity (μmol/min): With No Inhibitor: 10.4, 14.5, 22.5, 33.8, 40.5
Velocity (μmol/min) With Inhibitor: 4.1, 6.4, 11.3, 22.6, 33.8
(a) What are the values of Vmax and Km in the absence of inhibitor? In its presence?