Put the following events in the construction of a recombinant DNA molecule in the correct order: a. ligation of desired gene to plasmid DNA b. introduction of the plasmid into bacteria c. restriction enzymes cut gene of interest and plasmid DNA d. growth of cells on an antibiotic-containing medium

C, A, B, D

Explanation:

Recombinant DNA contains genetic information from more than one source. It is widely used for amplification of particular genes in biotechnology. The steps for recombinant DNA technology are;

1) The restriction enzymes cut the specific gene of interest and paste it in plasmid.

2) The DNA ligase joins the strands.

3) Plasmid is inserted into bacteria.

4) Antibiotic selection is used to identify recombinant plasmids.

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1. b.

2. d.

3. a.

5. d.

6. c.

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