To study how proteins fold, scientists must be able to purify the protein of interest, use solvents to denature the folded protein, and observe the process of refolding at successive time points. What is the effect of the solvents used in the denaturation process?(a) The solvents break all covalent interactions.
(b) The solvents break all noncovalent interactions.
(c) The solvents break some of the noncovalent interactions, resulting in a misfolded protein.
(d) The solvents create a new protein conformation.

B) The solvent breaks all non covalent interactions.

Explanation:

Proteins re made up of polypeptide chains formed from sequences of amino acids.

To form a polypeptide the amino acid are attached to each other by a covalent peptide bond.

In order to form the 3D conformation, the proteins structure is held together by inter-molecular forces that are non covalent.

Therefore for this study where the scientist wants to study  how proteins fold. The solvent used will break all non covalent interaction inorder to observe the refolding process.