A southern blot is a technique that relies on hybridization of

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A nucleic acid probe to a complementary DNA.

Explanation:

Southern blotting is a technique is used to detect the specific DNA fragment in the DNA sample. The DNA fragments are separated during the electrophoresis.

The process of electrophoresis separates the DNA fragments on the gel which is then kept over a sponge dipped in the buffer solution. Over the gel, the nitrocellulose sheet is placed over which the weight or pressure is applied. The buffer solution denatures the dsDNA and the ss DNA binds the nitrocellulose sheet. Now the sheet is hybridized with radiolabeled DNA probes specific to the target DNA. The probe binds the target DNA by complementary pairing rule which is the crucial step of the experiment.

Thus, the selected option is the correct answer.

step 1: initiation

initiation is the beginning of transcription. it occurs when the enzyme rna polymerase binds to a region of a gene called the promoter. this signals the dna to unwind so the enzyme can ‘‘read’’ the bases in one of the dna strands. the enzyme is now ready to make a strand of mrna with a complementary sequence of bases.

step 2: elongation

elongation is the addition of nucleotides to the mrna strand. rna polymerase reads the unwound dna strand and builds the mrna molecule, using complementary base pairs. there is a brief time during this process when the newly formed rna is bound to the unwound dna. during this process, an adenine (a) in the dna binds to an uracil (u) in the rna.

step 3: termination

termination is the ending of transcription, and occurs when rna polymerase crosses a stop (termination) sequence in the gene. the mrna strand is complete, and it detaches from dna.

b a mature ovary of a plant.