What were the first single-celled organisms like?

The first living things on Earth, single-celled micro-organisms or microbes lacking a cell nucleus or cell membrane known as prokaryotes, seem to have first appeared on Earth almost four billion years ago, just a few hundred million years after the formation of the Earth itself.

an active site is a region that is present on an enzyme where the reactants or the substrate molecules we can say bind and experience a chemical reaction. the substrate is basically a reactant whose concentration is changing and that is converting into a product after binding at the active site of an enzyme