You want to study the oxygen binding ability of hemoglobin at ph 7.21 and ph 7.60. you decide to use a phosphate buffer.

a) how would you make up 500 ml of 50 mm phosphate buffer, ph 7.21, starting with 1.00 m h3po4 and either 10.0 m hcl or 10.0 m naoh?

b) how would you make up 500 ml of 50 mm phosphate buffer, ph 7.60, starting from your 50mm phosphate buffer at ph 7.21?